Fish culturists have long sought for methods to secure year-round availability of gametes from seasonal spawning fish. Spermatozoa of several teleost species have been stored for long periods at liquid gas temperatures, but eggs, whether fertilized or unfertilized, have been considered unlikely candidates for cryopreservation. This paper describes some brief cooling trials to assess the possibilities for the cryopreservation of plaice eggs (Pleuronectesplatessa), and also reports on some limited progress with storage at near-zero temperatures using an apparatus with electronic temperature control.
Progress in storing marine flatfish eggs at low temperature.
Pullin, R.S.V., Bailey, H. (1981)
Rapports et Procès-Verbaux des Réunions CIEM 178: 514-517